Urinary oxalate by rate analysis compared with gas-chromatographic and centrifugal analyzer methods.
نویسندگان
چکیده
منابع مشابه
An enzymatic method for oxalate automated with the Cobas Fara centrifugal analyzer.
Measurement of oxalate in urine has been automated for use with the Cobas Fara centrifugal analyzer. No sample pretreatment other than (a critical) pH adjustment is required. Between-run CVs were less than 4%. Results were linearly related to oxalate concentration to 1000 mumol/L. Ascorbic acid ingestion, up to 5 g of ascorbate daily, caused no demonstrable interference with the assay. This pra...
متن کاملPreventing ascorbate interference in ion-chromatographic determinations of urinary oxalate: four methods compared.
In an attempt to decrease ascorbate interference on the ion-chromatographic determination of urinary oxalate, we compared the effectiveness of four different methods for ascorbate elimination by analyzing a representative urine pool supplemented with successive ascorbate additions. Two of the methods--treatment with ferric ions or boric acid--have been described elsewhere; treatments with nitri...
متن کاملMeasurement of urinary oxalate: an enzymatic and an ion chromatographic method compared.
An ion chromatographic and an enzymatic method for determination of oxalic acid in urine were compared on the basis of reliability and practicability. Both methods displayed a within-run imprecision of under 5% and a total imprecision of under 10%. The mean recovery of 0.2 mmol/l sodium oxalate lay close to 100% for both methods. After 80 parallel determinations, both methods produced statistic...
متن کاملSimple, reliable chromatographic measurement of oxalate in urine.
In this assay for oxalate in urine, oxalate is adsorbed from the urine onto graphitized carbon black (Carbopack B). After desorption and removal of the solvent, oxalic acid is gas-chromatographically measured after being derivatized with BF3/methanol. Chromatography is on Carbopack B/polyethylene glycol (Mr 20000), 93.7/6.3 by weight. The lower limit of detection of urinary oxalate is about 3 m...
متن کاملGas-chromatographic determination of urinary oxalate.
We describe a simple, rapid method for determining urinary oxalate: isolation by precipitation with calcium chloride and conversion to dimethyl oxalate, which then is measured by gas chromatography. To each sample, tracer amounts of 14C-labeled oxalic acid are added, to determine the analytical recovery of urinary oxalate. Analytical recovery of [14C]oxalic acid added to urine specimens ranged ...
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عنوان ژورنال:
- Clinical chemistry
دوره 30 11 شماره
صفحات -
تاریخ انتشار 1984